Amniotic fluid phospholipid profile.

Critical assessment of phospholipid measurement in amniotic fluid.

We assessed several methods of inorganic phosphate assay for their suitability in estimating phospholipids in digested extracts of amniotic fluids. The extraction and digestion procedures used for phospholipids from amniotic fluid were also examined critically. The effect of contamination by blood or obstetric cream has been examined. Accordingly, we suggest a method for measuring total phospho...

Amniotic fluid phospholipid profile determined by two-dimensional thin-layer chromatography as index of fetal lung maturation.

A phospholipid profile, the main features of which were the lecithin/sphingomyelin (L/S) ratio and the presence or absence of phosphatidylglycerol (PG), was determined in amniotic fluid from 188 patients. There was a mature profile (L/S ratio of at least 2 . 0 and detectable PG) in 145 patients, including seven insulin-dependent diabetics, and noe of their babies developed respiratory distress ...

The Comparison of Maternal Endometritis in Meconium – Stained Amniotic Fluid and Clear Amniotic Fluid

Introduction: Metritis is one of the causes of maternal morbidity and there are many risk factors for it. Because of the importance of possible association of meconium-stained amniotic fluid and maternal metritis the present study was done to determine whether meconium stained amniotic fluid (MSAF) is associated with puerperal infection. Methods: In this case-control study 150 women with MSAF a...

Amniotic fluid cell cultures.

In an 18-month period, 249 samples of amniotic fluid were used experimentally in attempts to enhance success in cell cultures. None ofthe experimental situations proved entirely satisfactory in this aim. Emphasis is laid on the quality ofserum used in culture. Particularly satisfactory serum should be retained for use in amniotic fluid cell cultures which are dependent on unknown growth stimula...

Amniotic Fluid Testing